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After final alkylation, all samples were diluted (1:1) with 20% ACN/0.1% FA containing 50 nM of deuterated internal standards and analyzed by LC-MS

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This work was supported by the Shandong Province Key R&D Program (Major Science and Technology Innovation Project, 2021CXGC010509), Key Laboratory of Chronic Deficiency Liver Disease of the State Administration of Traditional Chinese Medicine of the People’s Republic of China (20DZ2272200), Shanghai Key Specialty of Traditional Chinese Clinical Medicine (shslczdzk01201)