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BPC 157+ TB 500 – Limitless Wellness Lab

Crude protein extracts from mycelia were obtained by extraction from ground mycelia with B250 buffer (250 mM NaCl, 100 mM Tris–HCl pH 7.5, 10% glycerol, 1 mM EDTA and 0.1% NP-40) supplemented with 1.5 mL/L 1 M DTT, 2 tablets/100 mL complete-mini protease inhibitor cocktail EDTA-free (Roche), 3 mL/L 0.5 M Benzamidine, 10 ml/L phosphatase inhibitors 100× (10 M NaF, 5 M Na Vanadate, 8 M β- glycerol phosphate), and 10 mL/L 100 mM PMSF

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Bioinformatics 30 (Issue 9), 1312–1313