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However, pharmacokinetic parameters of the gastrodin metabolite 4-HBA changed significantly: antibiotic treatment reduced 4-HBA Cmax by 40.5% and AUC by 34.3%, while volume of distribution (Vd) and clearance (CL) increased by 100% and 55.2%, respectively, indicating that intestinal microbiota inhibition significantly reduces conversion of gastrodin to 4-HBA (Nepal et al., 2019)

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CBS is a cytosolic homotetrametic enzyme of about 63 kDa subunits that binds two cofactors, pyridoxal 5′‐phosphate (PLP) and haem (Meier et al ., 2001)