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The supernatant of protein extracts was heated at 70 °C for 10 min in SDS loading buffer (2% (w/v) SDS, 10% (w/v) glycerol, 0.01% (w/v) bromophenol blue and 0.125 M Tris-HCl, 0.3 M DTT, pH 6.8) and loaded on SDS-PAGE acrylamide gels for western blotting

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