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Unlike amine-reactive methods, where hydroxyl groups on the support are generally used, most sulfhydryl-reactive methods require the introduction of an amine,carboxyl group, or some other intermediate site onto the support

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These changes were accompanied by strong metabolic differences in the faeces, caecal content, liver and gut tissues between TCDF-treated and control AhR +/+ mice, whereas TCDF had no effect on the metabolic profiles of AhR −/− mice