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Invitrogen SuperScript IV VILO Master Mix (Thermo Fisher Scientific) was employed to convert 250 ng of total RNA into cDNA as per the manufacturer’s instructions, including prior genomic DNA removal with kit-supplied ezDNase

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Clinical trial data do not apply: Eli Lilly’s investigational Retatrutide has been studied in human clinical trials, and adverse event data from those trials are published [3]

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