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Glutathione is an essential antioxidant and sulfur compound that’s composed of three amino acids—cysteine, glycine, and glutamic acid—and is the major non-protein thiol in most organisms, including humans

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In order to determine the minimal concentrations of each protein required to achieve robust assay results, one should first perform a matrix titration in a 1536-well plate to systematically determine the assay signal from different concentrations of each protein in assay buffer with a fixed concentration of AlphaScreen reagents