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K11-Ub antibody production The amino acid sequence of the K11-Ub linkage-specific 2A3/2E6 antibody was as previously described 60 , and the coding sequence was synthesized by Thermo Scientific and cloned into the pVITRO plasmid using AgeI, BsrGI, BspeI and BamHI restriction enzymes (New England Biolabs)